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CHD4 antibody

Anti-CHD4 antibody used in Immunoprecipitation (IP). GTX101662
Anti-CHD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101662
Anti-CHD4 antibody used in Western Blot (WB). GTX101662

Cat No. GTX101662

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

218 kDa. ( Note )

Positive Control

Jurkat , Raji , K562

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CHD4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

chromodomain helicase DNA binding protein 4,CHD-4,Mi-2b,Mi2-BETA,SIHIWES

Cellular Localization

Nucleus

Background

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-CHD4 antibody used in Immunoprecipitation (IP). GTX101662

GTX101662 IP Image

Immunoprecipitation of CHD4 protein from 293T whole cell extracts using 5 μg of CHD4 antibody (GTX101662).
Western blot analysis was performed using CHD4 antibody (GTX101662).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CHD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101662

GTX101662 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CHD4(GTX101662) antibody at 1:500 dilution.

Anti-CHD4 antibody used in Western Blot (WB). GTX101662

GTX101662 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
5% SDS PAGE
GTX101662 diluted at 1:1000

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169