Immunoprecipitation of CHD4 protein from 293T whole cell extracts using 5 μg of CHD4 antibody (GTX101662).
Western blot analysis was performed using CHD4 antibody (GTX101662) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD4 antibody (GTX101662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CHD4 antibody detects CHD4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
CHD4 stained by CHD4 antibody (GTX101662) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CHD4 antibody detects CHD4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
CHD4 stained by CHD4 antibody (GTX101662) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CHD4 antibody detects CHD4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
CHD4 stained by CHD4 antibody (GTX101662) diluted at 1:25.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CHD4 antibody detects CHD4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
CHD4 stained by CHD4 antibody (GTX101662) diluted at 1:25.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CHD4 antibody detects CHD4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CHD4 stained by CHD4 antibody (GTX101662) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).