ICC: Use at a dilution of 1/200. IP: Use at a dilution of 1/200. WB: Use at a dilution of 1/500. Predicted molecular weight: 106 kDa. Optimal dilutions/concentrations should be determined by the end user.
CHERP mRNA is expressed ubiquitously in various tissues including lung, brain, heart, pancreas, kidney and skeletal muscle.
Antibody stabilization buffer.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.75 mg/ml (Please refer to the vial label for the specific concentration.)
Synthetic peptides, post synthetically modified to achieve desired antigenicty.
Affinity purified against immobilised antigen.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Calcium Homeostasis Endoplasmic Reticulum Protein,Dan16,Scaf6,Sra1,Cherp
The Calcium homoeostasis endoplasmic reticulum protein (CHERP) gene has been recently identified and localized on chromosome 19p13.1. CHERP has high homology to an SR related CTD protein, which is known to interact with the largest subunit of RNA polymerase II. CHERP is an integral endoplasmic reticulum membrane protein is involved in calcium mobilization induced by thrombin. Confocal microscopy revealed that CHERP is associated with Ins(1,4,5)P3 receptor throughout the cytoplasm and perinuclear region in Jurkat T lymphocyte. CHERP Anti sense treatment induced a decrease in CHERP, whichv caused an impaired increase in free cytoplasmic calcium but calcium influx remains unaffected, with some deficient in endoplasmic reticulum calcium stores. In the CHERP depleted Jurkat T lymphocytes the calcium dependent translocation of nuclear factor of activated T cells (NFAT) from cytoplasm to nucleus was also suppressed with significant suppression of cell.