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CHMP2B antibody

Anti-CHMP2B antibody used in IHC (Paraffin sections) (IHC-P). GTX118181
Anti-CHMP2B antibody used in Western Blot (WB). GTX118181
Anti-CHMP2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118181
Anti-CHMP2B antibody used in Western Blot (WB). GTX118181

Cat No. GTX118181

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

293T , A431

Predict Reactivity

Mouse, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CHMP2B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174469

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

charged multivesicular body protein 2B , ALS17 , CHMP2.5 , DMT1 , VPS2-2 , VPS2B

Cellular Localization

Cytoplasm , cytosol , Late endosome membrane

Background

This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CHMP2B antibody used in IHC (Paraffin sections) (IHC-P). GTX118181

GTX118181 IHC-P Image

CHMP2B antibody detects CHMP2B protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CHMP2B antibody (GTX118181) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CHMP2B antibody used in Western Blot (WB). GTX118181

GTX118181 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
12% SDS PAGE
GTX118181 diluted at 1:1000

Anti-CHMP2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118181

GTX118181 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using CHMP2B(GTX118181) antibody at 1:500 dilution.

Anti-CHMP2B antibody used in Western Blot (WB). GTX118181

GTX118181 WB Image

Jurkat whole cell extracts and Jurkat exosome extract (15.4 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP2B antibody (GTX118181) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf