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CHMP2B antibody

Orthogonal
Anti-CHMP2B antibody used in Western Blot (WB). GTX118181
Anti-CHMP2B antibody used in IHC (Paraffin sections) (IHC-P). GTX118181
Anti-CHMP2B antibody used in Western Blot (WB). GTX118181
Anti-CHMP2B antibody used in Western Blot (WB). GTX118181
Anti-CHMP2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118181
Anti-CHMP2B antibody used in Western Blot (WB). GTX118181

Cat. No. GTX118181

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CHMP2B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

293T , A431

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CHMP2B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174469

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

charged multivesicular body protein 2B , ALS17 , CHMP2.5 , DMT1 , VPS2-2 , VPS2B

Cellular Localization

Cytoplasm , cytosol , Late endosome membrane

Background

This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CHMP2B antibody used in Western Blot (WB). GTX118181

GTX118181 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP2B antibody (GTX118181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CHMP2B antibody used in IHC (Paraffin sections) (IHC-P). GTX118181

GTX118181 IHC-P Image

CHMP2B antibody detects CHMP2B protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CHMP2B antibody (GTX118181) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CHMP2B antibody used in Western Blot (WB). GTX118181

GTX118181 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX118181 diluted at 1:1000

Anti-CHMP2B antibody used in Western Blot (WB). GTX118181

GTX118181 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
12% SDS PAGE
GTX118181 diluted at 1:1000

Anti-CHMP2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118181

GTX118181 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using CHMP2B(GTX118181) antibody at 1:500 dilution.

Anti-CHMP2B antibody used in Western Blot (WB). GTX118181

GTX118181 WB Image

Orthogonal

Jurkat whole cell extracts and Jurkat exosome extract (15.4 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP2B antibody (GTX118181) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Alshafie W et al. F1000Res 2023; 12 : 884 The identification of high-performing antibodies for Charged multivesicular body protein 2b for use in Western Blot, immunoprecipitation and immunofluorescence.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169