GeneTex
United States (US)

CHMP4B antibody

GTX64853_20180530_IHC-P_2_18121411_332.jpg
GTX64853_20180530_WB_18121411_751.jpg
GTX64853_20180530_IHC-P_18121411_740.jpg
GTX64853_20180530_IHC-P_1_18121411_441.jpg

Cat No. GTX64853

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

25 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-224 of human CHMP4B (NP_789782.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CTPP3 Antibody , VPS32B Antibody , C20ORF178 Antibody , CHMP4A Antibody , DJ553F4.4 Antibody , SNF7 Antibody , VPS322 Antibody , SNF72 Antibody , SHAX1 Antibody

Background

This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies. Mutations in this gene result in autosomal dominant posterior polar cataracts.[provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES
GTX64853_20180530_IHC-P_2_18121411_332.jpg

GTX64853 IHC-P Image

IHC-P analysis of rat lung tissue using GTX64853 CHMP4B antibody.
Dilution : 1:100

GTX64853_20180530_WB_18121411_751.jpg

GTX64853 WB Image

WB analysis of various samples using GTX64853 CHMP4B antibody.The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

GTX64853_20180530_IHC-P_18121411_740.jpg

GTX64853 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX64853 CHMP4B antibody.
Dilution : 1:100

GTX64853_20180530_IHC-P_1_18121411_441.jpg

GTX64853 IHC-P Image

IHC-P analysis of human rectum tissue using GTX64853 CHMP4B antibody.
Dilution : 1:100