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CHST2 antibody [N3C3]

Anti-CHST2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119099
Anti-CHST2 antibody [N3C3] used in Western Blot (WB). GTX119099

Cat. No. GTX119099

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CHST2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CHST2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166295

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carbohydrate sulfotransferase 2 , C6ST , GST-2 , GST2 , Gn6ST-1 , HEL-S-75 , glcNAc6ST-1

Cellular Localization

Golgi apparatus , trans-Golgi network membrane

Background

N-acetylglucosamine-6-O-sulfotransferases, such as CHST2, catalyze the transfer of sulfate from 3-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) to position 6 of a nonreducing N-acetylglucosamine (GlcNAc) residue (Uchimura et al., 1998 [PubMed 9722682]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CHST2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119099

GTX119099 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using CHST2(GTX119099) antibody at 1:500 dilution.

Anti-CHST2 antibody [N3C3] used in Western Blot (WB). GTX119099

GTX119099 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX119099 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169