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CHX10 antibody

Anti-CHX10 antibody used in IHC (Paraffin sections) (IHC-P). GTX114143

Cat. No. GTX114143

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Mouse, Chicken
Package
100 μl ($399),
25 μl ($169)
See all CHX10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Predict Reactivity

Human, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CHX10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11161878

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

visual system homeobox 2 , CHX10 , HOX10 , MCOP2 , MCOPCB3 , RET1

Cellular Localization

Nucleus

Background

This gene encodes a homeobox protein originally described as a retina-specific transcription factor. Mutations in this gene are associated with microphthalmia, cataracts and iris abnormalities. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CHX10 antibody used in IHC (Paraffin sections) (IHC-P). GTX114143

GTX114143 IHC-P Image

CHX10 antibody detects CHX10 protein by immunohistochemical analysis.
Samples: Paraffin-embedded mouse retina.
Green: CHX10 protein stained by CHX10 antibody (GTX114143) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

An MJ et al. iScience 2024; 27 (8) : 110380 Reorganization of H3K9me heterochromatin leads to neuronal impairment via the cascading destruction of the KDM3B-centered epigenomic network.
Yamagata M et al. Elife 2021; 10 A cell atlas of the chick retina based on single-cell transcriptomics.
Application : ICC/IF
Reactivity : Chicken
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169