APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , K562 , THP-1 , HL-60 , NCI-H929 , NIH-3T3 , JC , BCL-1
Predict Reactivity
Bovine, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human CIAPIN1 Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11179152
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cytokine induced apoptosis inhibitor 1 , Anamorsin , CIAE2 , DRE2 , PRO0915
Cellular Localization
Cytoplasm
Background
CIAPIN1 is a cytokine-induced inhibitor of apoptosis with no relation to apoptosis regulatory molecules of the BCL2 (MIM 151430) or CASP (see MIM 147678) families. Expression of CIAPIN1 is dependent on growth factor stimulation (Shibayama et al., 2004 [PubMed 14970183]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX111212 ICC/IF Image
CIAPIN1 antibody detects CIAPIN1 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CIAPIN1 protein stained by CIAPIN1 antibody (GTX111212) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX111212 IHC-P Image
CIAPIN1 antibody detects CIAPIN1 protein at cytosol and nucleus on SkHep1 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SkHep1 xenograft.
CIAPIN1 antibody (GTX111212) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX111212 WB Image
Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
D: THP-1
E: HL-60
F: NCI-H929
10% SDS PAGE
GTX111212 diluted at 1:1000
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GTX111212 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX111212 diluted at 1:3000
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GTX111212 IHC-P Image
CIAPIN1 antibody detects CIAPIN1 protein at cytoplasm and nucleus in rat adrenal gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat adrenal gland.
CIAPIN1 antibody (GTX111212) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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