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CIDEC antibody

Anti-CIDEC antibody used in IHC (Paraffin sections) (IHC-P). GTX116733
Anti-CIDEC antibody used in Western Blot (WB). GTX116733
Anti-CIDEC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116733

Cat. No. GTX116733

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

HeLa , HepG2

Predict Reactivity

Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CIDEC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171373

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell death inducing DFFA like effector c , CIDE-3 , CIDE3 , FPLD5 , FSP27

Cellular Localization

Cytoplasm

Background

DNA fragmentation factor (DFF) induces the fragmentation of DNA associated with apoptosis. A novel family of cell death-inducing DFF45 (MIM 601882)-like effectors (CIDEs), including CIDEC, can also promote apoptosis (Liang et al., 2003 [PubMed 12429024]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CIDEC antibody used in IHC (Paraffin sections) (IHC-P). GTX116733

GTX116733 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using CIDEC(GTX116733) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CIDEC antibody used in Western Blot (WB). GTX116733

GTX116733 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: HepG2
12% SDS PAGE
GTX116733 diluted at 1:1000

Anti-CIDEC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116733

GTX116733 ICC/IF Image

CIDEC antibody detects CIDEC protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CIDEC protein stained by CIDEC antibody (GTX116733) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169