GeneTex
United States (US)

CIP29 antibody [N1C3]

GTX106633_41017_IFA_19011506_895.jpg
GTX106633_WB_19011506_923.jpg
GTX106633_IHC_19011506_240.jpg

Cat No. GTX106633

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CIP29. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CIP29 antibody, HCC1 antibody, HSPC316 antibody, MGC14726 antibody, THO1 antibody, SARNP antibody, nuclear protein Hcc-1 antibody, SAP domain-containing ribonucleoprotein antibody, cytokine-induced protein of 29 kDa antibody, hepatocellular carcinoma 1 antibody, cytokine induced protein 29 kDa antibody, proliferation-associated cytokine-inducible protein CIP29 antibody, proliferation associated cytokine-inducible protein CIP29 antibody, SAP domain containing ribonucleoprotein antibody

Cellular Localization

Nucleus

Background

This gene encodes a protein that is upregulated in response to various cytokines. The encoded protein may play a role in cell cycle progression. A translocation between this gene and the myeloid/lymphoid leukemia gene, resulting in expression of a chimeric protein, has been associated with acute myelomonocytic leukemia. Pseudogenes exist on chromosomes 7 and 8. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Database

DATA IMAGES
GTX106633_41017_IFA_19011506_895.jpg

GTX106633 ICC/IF Image

CIP29 antibody [N1C3] detects CIP29 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CIP29 protein stained by CIP29 antibody [N1C3] (GTX106633) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX106633_WB_19011506_923.jpg

GTX106633 WB Image

Sample(30 ug whole cell lysate)
A:293T
B:A431(GTX27909)
C:H1299
12% SDS PAGE
GTX106633 diluted at 1:1000

GTX106633_IHC_19011506_240.jpg

GTX106633 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB-468 xenograft, using CIP29(GTX106633) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169