GeneTex
United States (US)

CIS antibody [C2C3], C-term

GTX100216_39391_WB_R_19011506_926.jpg
GTX100216_IHC_19011506_130.jpg
GTX100216_39391_IFA_19011506_253.jpg
GTX100216_39391_WB_19011506_216.jpg

Cat No. GTX100216

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

HeLa , PC-12 , Rat2

Predict Reactivity

Mouse, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CIS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CIS antibody, CIS-1 antibody, G18 antibody, SOCS antibody, CISH antibody, suppressor of cytokine signaling antibody, cytokine-inducible inhibitor of signaling type 1B antibody, cytokine-inducible SH2-containing protein antibody, cytokine inducible SH2-containing protein antibody

Background

The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100216_39391_WB_R_19011506_926.jpg

GTX100216 WB Image

CIS antibody [C2C3], C-term detects CISH protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
CIS antibody [C2C3], C-term (GTX100216) dilution: 1:1000

GTX100216_IHC_19011506_130.jpg

GTX100216 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using CIS(GTX100216) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100216_39391_IFA_19011506_253.jpg

GTX100216 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using CIS(GTX100216) antibody at 1:200 dilution.

GTX100216_39391_WB_19011506_216.jpg

GTX100216 WB Image

Sample (1 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX100216 diluted at 1:10000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169