GeneTex
United States (US)

CIZ1 antibody [N2C1], Internal

GTX102144_40163_IP_19011506_444.jpg
GTX102144_40163_IFA_19011506_700.jpg
GTX102144_40163_IHC_19011506_874.jpg
GTX102144_40163_WB_19011506_252.jpg

Cat No. GTX102144

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CIZ1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

LSFR1 antibody, NP94 antibody, ZNF356 antibody, CIZ1 antibody, CDKN1A-interacting zinc finger protein 1 antibody, nuclear protein NP94 antibody, zinc finger protein 356 antibody, cip1-interacting zinc finger protein antibody, CDKN1A interacting zinc finger protein 1 antibody

Cellular Localization

Nucleus

Background

May regulate the subcellular localization of CIP/WAF1.

Database

DATA IMAGES
GTX102144_40163_IP_19011506_444.jpg

GTX102144 IP Image

CIZ1 antibody immunoprecipitates CIZ1 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of CIZ1 protein by 2.5 μg of CIZ1 antibody (GTX102144) 7.5% SDS-PAGE The immunoprecipitated CIZ1 protein was detected by CIZ1 antibody (GTX102144) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX102144_40163_IFA_19011506_700.jpg

GTX102144 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CIZ1(GTX102144) antibody at 1:200 dilution.

GTX102144_40163_IHC_19011506_874.jpg

GTX102144 IHC-P Image

Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CIZ1(GTX102144) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102144_40163_WB_19011506_252.jpg

GTX102144 WB Image

CIZ1 antibody [N2C1], Internal detects CIZ1 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
7.5 % SDS-PAGE
CIZ1 antibody [N2C1], Internal (GTX102144) dilution: 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169