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CIZ1 antibody [N2C1], Internal

Anti-CIZ1 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX102144
Anti-CIZ1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102144
Anti-CIZ1 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102144
Anti-CIZ1 antibody [N2C1], Internal used in Western Blot (WB). GTX102144

Cat No. GTX102144

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CIZ1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CDKN1A interacting zinc finger protein 1 , LSFR1 , NP94 , ZNF356

Cellular Localization

Nucleus

Background

May regulate the subcellular localization of CIP/WAF1.

Database

Research Area

DATA IMAGES

Anti-CIZ1 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX102144

GTX102144 IP Image

CIZ1 antibody immunoprecipitates CIZ1 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of CIZ1 protein by 2.5 μg of CIZ1 antibody (GTX102144) 7.5% SDS-PAGE The immunoprecipitated CIZ1 protein was detected by CIZ1 antibody (GTX102144) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CIZ1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102144

GTX102144 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CIZ1(GTX102144) antibody at 1:200 dilution.

Anti-CIZ1 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102144

GTX102144 IHC-P Image

Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CIZ1(GTX102144) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CIZ1 antibody [N2C1], Internal used in Western Blot (WB). GTX102144

GTX102144 WB Image

CIZ1 antibody [N2C1], Internal detects CIZ1 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
7.5 % SDS-PAGE
CIZ1 antibody [N2C1], Internal (GTX102144) dilution: 1:1000

REFERENCE

There are currently no references for CIZ1 antibody [N2C1], Internal (GTX102144). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CIZ1 antibody [N2C1], Internal (GTX102144). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169