CIZ1 antibody [N2C1], Internal

Cat. No. GTX102144

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all CIZ1 products

Datasheet
Document

View product citations for antibody GTX102144 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CIZ1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615370

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CDKN1A interacting zinc finger protein 1 , LSFR1 , NP94 , ZNF356

Cellular Localization

Nucleus

Background

May regulate the subcellular localization of CIP/WAF1.

Database

Gene ID: 25792 CIZ1
UniProt: Q9ULV3 CIZ1

Research Area

DATA IMAGES

GTX102144 IP Image

CIZ1 antibody immunoprecipitates CIZ1 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of CIZ1 protein by 2.5 μg of CIZ1 antibody (GTX102144) 7.5% SDS-PAGE The immunoprecipitated CIZ1 protein was detected by CIZ1 antibody (GTX102144) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX102144 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CIZ1(GTX102144) antibody at 1:200 dilution.

GTX102144 WB Image

CIZ1 antibody [N2C1], Internal detects CIZ1 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
7.5 % SDS-PAGE
CIZ1 antibody [N2C1], Internal (GTX102144) dilution: 1:1000

GTX102144 IHC-P Image

Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CIZ1(GTX102144) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty