APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3
Predict Reactivity
Rat, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.43 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CKAP1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Tubulin Folding Cofactor B,Cg22,Ckap1,Ckapi,Tbcb
Cellular Localization
Cytoplasm , cytoskeleton
Background
Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth.
Database
DATA IMAGES
|
GTX101940 WB Image
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
12% SDS PAGE
GTX101940 diluted at 1:1000
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GTX101940 ICC/IF Image
Immunofluorescence analysis of methanol-fixed A549, using CKAP1(GTX101940) antibody at 1:500 dilution.
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GTX101940 IHC-P Image
Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using CKAP1(GTX101940) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101940 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
GTX101940 diluted at 1:1000
|
