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CKAP1 antibody [N1C3]

Anti-CKAP1 antibody [N1C3] used in Western Blot (WB). GTX101940
Anti-CKAP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101940
Anti-CKAP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101940
Anti-CKAP1 antibody [N1C3] used in Western Blot (WB). GTX101940

Cat No. GTX101940

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all CKAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CKAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin folding cofactor B , CG22 , CKAP1 , CKAPI

Cellular Localization

Cytoplasm , cytoskeleton

Background

Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth.

Database

Research Area

DATA IMAGES

Anti-CKAP1 antibody [N1C3] used in Western Blot (WB). GTX101940

GTX101940 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
12% SDS PAGE
GTX101940 diluted at 1:1000

Anti-CKAP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101940

GTX101940 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using CKAP1(GTX101940) antibody at 1:500 dilution.

Anti-CKAP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101940

GTX101940 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using CKAP1(GTX101940) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CKAP1 antibody [N1C3] used in Western Blot (WB). GTX101940

GTX101940 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
GTX101940 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169