GeneTex
United States (US)

CKAP1 antibody [N1C3]

GTX101940_WB_19011506_907.jpg
GTX101940_40135_IFA_19011506_603.jpg
GTX101940_IHC_19011506_983.jpg
GTX101940_40135_WB_M_19011506_681.jpg

Cat No. GTX101940

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CKAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tubulin Folding Cofactor B,Cg22,Ckap1,Ckapi,Tbcb

Cellular Localization

Cytoplasm , cytoskeleton

Background

Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth.

Database

DATA IMAGES
GTX101940_WB_19011506_907.jpg

GTX101940 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
12% SDS PAGE
GTX101940 diluted at 1:1000

GTX101940_40135_IFA_19011506_603.jpg

GTX101940 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using CKAP1(GTX101940) antibody at 1:500 dilution.

GTX101940_IHC_19011506_983.jpg

GTX101940 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using CKAP1(GTX101940) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101940_40135_WB_M_19011506_681.jpg

GTX101940 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
GTX101940 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169