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CKAP2 antibody

Anti-CKAP2 antibody used in Western Blot (WB). GTX110830
Anti-CKAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110830

Cat No. GTX110830

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

Raji , NCI-H929

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CKAP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885292

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cytoskeleton associated protein 2 , LB1 , TMAP , se20-10

Cellular Localization

Cytoplasm

Background

CKAP2 is a cytoskeleton-associated protein involved in mitotic progression (Seki and Fang, 2007 [PubMed 17376772]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CKAP2 antibody used in Western Blot (WB). GTX110830

GTX110830 WB Image

CKAP2 antibody detects CKAP2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP2 antibody (GTX110830) diluted by 1:1000.

Anti-CKAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110830

GTX110830 ICC/IF Image

CKAP2 antibody detects CKAP2 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CKAP2 protein stained by CKAP2 antibody (GTX110830) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169