Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP4 antibody [N3C3] (GTX120087) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CKAP4 antibody [N3C3] detects CKAP4 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CKAP4 stained by CKAP4 antibody [N3C3] (GTX120087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using CKAP4(GTX120087) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

CKAP4 antibody [N3C3] detects CKAP4 protein at cytoplasm and membrane in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
CKAP4 antibody [N3C3] (GTX120087) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CKAP4 antibody [N3C3] detects CKAP4 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CKAP4 stained by CKAP4 antibody [N3C3] (GTX120087) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP4 antibody [N3C3] (GTX120087) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.