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CKAP5 antibody

Anti-CKAP5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66531
Anti-CKAP5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66531
Anti-CKAP5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66531
Anti-CKAP5 antibody used in Western Blot (WB). GTX66531
Anti-CKAP5 antibody used in Immunoprecipitation (IP). GTX66531

Cat. No. GTX66531

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CKAP5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

225 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1763-2032 of human CKAP5 (NP_001008938.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytoskeleton associated protein 5 , CHTOG , MSPS , TOG , TOGp , ch-TOG

Background

This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CKAP5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66531

GTX66531 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX66531 CKAP5 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CKAP5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66531

GTX66531 ICC/IF Image

ICC/IF analysis of L929 cells using GTX66531 CKAP5 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CKAP5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66531

GTX66531 ICC/IF Image

ICC/IF analysis of H9C2 cells using GTX66531 CKAP5 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CKAP5 antibody used in Western Blot (WB). GTX66531

GTX66531 WB Image

WB analysis of various sample lysates using GTX66531 CKAP5 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CKAP5 antibody used in Immunoprecipitation (IP). GTX66531

GTX66531 IP Image

IP analysis of Jurkat cell lysate using GTX66531 CKAP5 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399