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CKII alpha antibody

Anti-CKII alpha antibody used in Western Blot (WB). GTX10474
Anti-CKII alpha antibody used in Immunoprecipitation (IP). GTX10474

Cat. No. GTX10474

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
50 μg ($399)
See all CKII alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:5000
IP 2 - 10 μg/mg lysate
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen recognized by this antibody maps to a region between residue 350 and the C-terminus (residue 350) of human Casein Kinase 2, alpha 2 using the numbering given in entry NP_001887.1 (GeneID 1459).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

casein kinase 2 alpha 2 , CK2A2 , CK2alpha'' , CSNK2A1

Background

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.

Database

Research Area

DATA IMAGES

Anti-CKII alpha antibody used in Western Blot (WB). GTX10474

GTX10474 WB Image

WB analysis of HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cell lysates in NETN lysis buffer using GTX10474 CKII alpha antibody.
Loading : 50μg
Dilution : 1 μg/ml

Anti-CKII alpha antibody used in Immunoprecipitation (IP). GTX10474

GTX10474 IP Image

IP analysis of HeLa cell lysate using GTX10474 CKII alpha antibody and CKII alpha' antibody.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399