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CKMT2 antibody [N2C3]

Anti-CKMT2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX111620
Anti-CKMT2 antibody [N2C3] used in Western Blot (WB). GTX111620
Anti-CKMT2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX111620

Cat. No. GTX111620

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CKMT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CKMT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949990

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

creatine kinase, mitochondrial 2 , SMTCK

Background

Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CKMT2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX111620

GTX111620 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using sMtCK(GTX111620) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CKMT2 antibody [N2C3] used in Western Blot (WB). GTX111620

GTX111620 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX111620 diluted at 1:1000

Anti-CKMT2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX111620

GTX111620 IHC-P Image

CKMT2 antibody [N2C3] detects CKMT2 protein at mitochondria in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
CKMT2 antibody [N2C3] (GTX111620) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169