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CLASP1 antibody

Anti-CLASP1 antibody used in Western Blot (WB). GTX115695
Anti-CLASP1 antibody used in Western Blot (WB). GTX115695
Anti-CLASP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115695
Anti-CLASP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115695

Cat. No. GTX115695

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CLASP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

169 kDa. ( Note )

Positive Control

HepG2 , Mouse brain

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CLASP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10622175

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytoplasmic linker associated protein 1 , MAST1

Cellular Localization

Cytoplasm , cytoskeleton , Centrosome , Kinetochore , Spindle

Background

CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170; MIM 179838). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle (Maiato et al., 2003 [PubMed 12837247]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CLASP1 antibody used in Western Blot (WB). GTX115695

GTX115695 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX115695 diluted at 1:1000

Anti-CLASP1 antibody used in Western Blot (WB). GTX115695

GTX115695 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
5% SDS PAGE
GTX115695 diluted at 1:1000

Anti-CLASP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115695

GTX115695 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using CLASP1(GTX115695) antibody at 1:500 dilution.

Anti-CLASP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115695

GTX115695 IHC-P Image

Immunohistochemical analysis of paraffin-embedded 4T1 xenograft, using CLASP1(GTX115695) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169