APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:100-1:1000 |
| 1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Serum
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Mouse
Immunogen
Immunogen: C-terminal peptide of mouse Calmegin, DESPGSGDAPLKSLRKRRVRKD, conjugated with KLH
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
calmegin , 4930459O04Rik , A2/6 , AI528775 , Cln
Cellular Localization
Endoplasmic reticulum membrane
Background
This gene belongs to the calreticulin family, which includes calreticulin, calnexin, and calmegin, and encodes a calcium-binding molecular chaperone specifically expressed in pachytene stage male germ cells. It is required for the proper folding of newly synthesized membrane proteins in the endoplasmic reticulum including those critical for sperm migration from the uterus into the oviduct and sperm adhesion to and penetration of the zona pellucida. This gene plays a key role in spermatogenesis and male infertility. Alternative splice variants exist for this gene. [provided by RefSeq, Jul 2016]
Database
Research Area
DATA IMAGES
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GTX00744 IP Image
IP analysis of mouse testis using GTX00744 CLGN antibody.
Total protein amount : 1 mg
IP antibody : 2 μg
IB antibody : 1:1000
Total protein amount : 1 mg
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GTX00744 IHC-P Image
IHC-P analysis of mouse testis tissue section using GTX00744 CLGN antibody.
Dilution : 1:1000
Antigen retrieval : Proteolytic-Induced Epitope Retrieval
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GTX00744 ICC/IF Image
ICC/IF analysis of NIH-3T3 cells using GTX00744 CLGN antibody.
Dilution : 1:100
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS for 10 min
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GTX00744 WB Image
WB analysis of mouse testis and sperm using GTX00744 CLGN antibody.
Dilution : 1:1000
Loading : 10 μg
SDS-PAGE : 10-20% gradent gel
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GTX00744 WB Image
WB analysis of wild-type and knockout mice using GTX00744 CLGN antibody.
(+/+) : CLGN+/+
(-/-) : GLGN-/-
Dilution : 1:1000
Loading : 20 μg
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REFERENCE
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REVIEW
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