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CLGN antibody

Anti-CLGN antibody used in Western Blot (WB). GTX00744
Anti-CLGN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00744
Anti-CLGN antibody used in Immunoprecipitation (IP). GTX00744
Anti-CLGN antibody used in Western Blot (WB). GTX00744
Anti-CLGN antibody used in IHC (Paraffin sections) (IHC-P). GTX00744

Cat No. GTX00744

Host

Rabbit

Clonality

Polyclonal

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Mouse
Package
100 μl ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:100-1:1000
IHC-P 1:1000
IP 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum with 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

Immunogen: C-terminal peptide of mouse Calmegin, DESPGSGDAPLKSLRKRRVRKD, conjugated with KLH

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calmegin , 4930459O04Rik , A2/6 , AI528775 , Cln

Cellular Localization

Endoplasmic reticulum membrane

Background

This gene belongs to the calreticulin family, which includes calreticulin, calnexin, and calmegin, and encodes a calcium-binding molecular chaperone specifically expressed in pachytene stage male germ cells. It is required for the proper folding of newly synthesized membrane proteins in the endoplasmic reticulum including those critical for sperm migration from the uterus into the oviduct and sperm adhesion to and penetration of the zona pellucida. This gene plays a key role in spermatogenesis and male infertility. Alternative splice variants exist for this gene. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-CLGN antibody used in Western Blot (WB). GTX00744

GTX00744 WB Image

WB analysis of wild-type and knockout mice using GTX00744 CLGN antibody.
(+/+) : CLGN+/+
(-/-) : GLGN-/-
Dilution : 1:1000
Loading : 20 μg

Anti-CLGN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00744

GTX00744 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX00744 CLGN antibody.
Dilution : 1:100
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS for 10 min

Anti-CLGN antibody used in Immunoprecipitation (IP). GTX00744

GTX00744 IP Image

IP analysis of mouse testis using GTX00744 CLGN antibody.
Total protein amount : 1 mg
IP antibody : 2 μg
IB antibody : 1:1000
Total protein amount : 1 mg

Anti-CLGN antibody used in Western Blot (WB). GTX00744

GTX00744 WB Image

WB analysis of mouse testis and sperm using GTX00744 CLGN antibody.
Dilution : 1:1000
Loading : 10 μg
SDS-PAGE : 10-20% gradent gel

Anti-CLGN antibody used in IHC (Paraffin sections) (IHC-P). GTX00744

GTX00744 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX00744 CLGN antibody.
Dilution : 1:1000
Antigen retrieval : Proteolytic-Induced Epitope Retrieval

REFERENCE

There are currently no references for CLGN antibody (GTX00744). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 359