WB analysis of wild-type and knockout mice using GTX00744 CLGN antibody.
(+/+) : CLGN^+/+
(-/-) : GLGN^-/-
Dilution : 1:1000
Loading : 20 μg

WB analysis of mouse testis and sperm using GTX00744 CLGN antibody.
Dilution : 1:1000
Loading : 10 μg
SDS-PAGE : 10-20% gradent gel

ICC/IF analysis of NIH-3T3 cells using GTX00744 CLGN antibody.
Dilution : 1:100
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS for 10 min

IHC-P analysis of mouse testis tissue section using GTX00744 CLGN antibody.
Dilution : 1:1000
Antigen retrieval : Proteolytic-Induced Epitope Retrieval

IP analysis of mouse testis using GTX00744 CLGN antibody.
Total protein amount : 1 mg
IP antibody : 2 μg
IB antibody : 1:1000
Total protein amount : 1 mg