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CLIC3 antibody [N1C3]

Anti-CLIC3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104397
Anti-CLIC3 antibody [N1C3] used in Western Blot (WB). GTX104397
Anti-CLIC3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104397

Cat. No. GTX104397

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CLIC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CLIC3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949996

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chloride intracellular channel 3

Cellular Localization

Nucleus , Membrane , Cytoplasm

Background

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 3 is a member of the p64 family and is predominantly localized in the nucleus and stimulates chloride ion channel activity. In addition, this protein may participate in cellular growth control, based on its association with ERK7, a member of the MAP kinase family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CLIC3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104397

GTX104397 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human T(IV)(lung CA), using CLIC3(GTX104397) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CLIC3 antibody [N1C3] used in Western Blot (WB). GTX104397

GTX104397 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
12% SDS PAGE
GTX104397 diluted at 1:500

Anti-CLIC3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104397

GTX104397 IHC-P Image

CLIC3 antibody [N1C3] detects CLIC3 protein at membrane and cytoplasm on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
CLIC3 antibody [N1C3] (GTX104397) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169