GeneTex
United States (US)

CLIC3 antibody [N1C3]

GTX104397_IHC1_19011506_239.jpg
GTX104397_WB_19011506_663.jpg
GTX104397_39883_20150116_IHC_M_19011506_206.jpg

Cat No. GTX104397

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CLIC3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Chloride Intracellular Channel 3,Clic3

Cellular Localization

Nucleus , Membrane , Cytoplasm

Background

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 3 is a member of the p64 family and is predominantly localized in the nucleus and stimulates chloride ion channel activity. In addition, this protein may participate in cellular growth control, based on its association with ERK7, a member of the MAP kinase family. [provided by RefSeq]

Database

DATA IMAGES
GTX104397_IHC1_19011506_239.jpg

GTX104397 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human T(IV)(lung CA), using CLIC3(GTX104397) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104397_WB_19011506_663.jpg

GTX104397 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
12% SDS PAGE
GTX104397 diluted at 1:500

GTX104397_39883_20150116_IHC_M_19011506_206.jpg

GTX104397 IHC-P Image

CLIC3 antibody [N1C3] detects CLIC3 protein at membrane and cytoplasm on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
CLIC3 antibody [N1C3] (GTX104397) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169