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CLIC4 antibody, N-term

Anti-CLIC4 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89013
Anti-CLIC4 antibody, N-term used in Western Blot (WB). GTX89013
Anti-CLIC4 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89013

Cat No. GTX89013

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μg ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
ICC/IF 5μg/ml
IHC-P 5μg/ml

Note :

ICC/IF
Min6 cells transiently expressing mouse Clic4 shows cytoplasmic staining of two cells.

Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89013-PEP).

Predict Reactivity

Rat, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence NGLKEEDKEPLIE-C, from the N Terminus of the protein sequence according to NP_039234.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Chloride Intracellular Channel 4,Clic4L,H1,Mtclic,Huh1,P64H1,Clic4

Cellular Localization

Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi netwo

Background

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-CLIC4 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89013

GTX89013 IHC-P Image

IHC-P analysis of human heart using GTX89013 CLIC4 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5µg/ml

Anti-CLIC4 antibody, N-term used in Western Blot (WB). GTX89013

GTX89013 WB Image

WB analysis of human kidney lysate using GTX89013 CLIC4 antibody, N-term.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

Anti-CLIC4 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89013

GTX89013 ICC/IF Image

ICC/IF analysis of min6 cells transiently expressing mouse Clic4 protein using GTX89013 CLIC4 antibody, N-term.
Dilution : 5µg/ml

REFERENCE
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REVIEW
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Package List Price ($)
$ 329