APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.3μg/ml |
| 5μg/ml |
| 5μg/ml |
|
Note :
ICC/IF
Min6 cells transiently expressing mouse Clic4 shows cytoplasmic staining of two cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89013-PEP).
Predict Reactivity
Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence NGLKEEDKEPLIE-C, from the N Terminus of the protein sequence according to NP_039234.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
chloride intracellular channel 4 , CLIC4L , H1 , MTCLIC , huH1 , p64H1
Cellular Localization
Cell membrane , Mitochondrion , Cell junction
Background
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89013 ICC/IF Image
ICC/IF analysis of min6 cells transiently expressing mouse Clic4 protein using GTX89013 CLIC4 antibody, N-term.
Dilution : 5μg/ml
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GTX89013 WB Image
WB analysis of human kidney lysate using GTX89013 CLIC4 antibody, N-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89013 IHC-P Image
IHC-P analysis of human heart using GTX89013 CLIC4 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
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REFERENCE
There are currently no references for CLIC4 antibody, N-term (GTX89013). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CLIC4 antibody, N-term (GTX89013). Be the first to share your experience with this product.
