Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CLIC4 antibody (GTX104888) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CLIC4 antibody (GTX104888) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using CLIC4(GTX104888) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using CLIC4 antibody (GTX104888) at 1:300 dilution.

Sample (30 μg of whole cell lysate)
A: whole zebrafish
12% SDS PAGE
GTX104888 diluted at 1:1000