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CLIC4 antibody

Anti-CLIC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104888
Anti-CLIC4 antibody used in Western Blot (WB). GTX104888
Anti-CLIC4 antibody used in Western Blot (WB). GTX104888

Cat No. GTX104888

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

HeLa , Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CLIC4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

chloride intracellular channel 4 , CLIC4L , H1 , MTCLIC , huH1 , p64H1

Cellular Localization

Cytoplasm , Cytoplasmic vesicle membrane; Single-pass membrane protein , Nucleus , Cell membrane; Single-pass membrane protein , Cytoplasm , Cytoplasmic vesicle membrane , Nucleus , Cell membrane

Background

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-CLIC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104888

GTX104888 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using CLIC4(GTX104888) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-CLIC4 antibody used in Western Blot (WB). GTX104888

GTX104888 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CLIC4 antibody (GTX104888) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CLIC4 antibody used in Western Blot (WB). GTX104888

GTX104888 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CLIC4 antibody (GTX104888) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169