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CLIP170 antibody [N1], N-term

Anti-CLIP170 antibody [N1], N-term used in Western Blot (WB). GTX117504
Anti-CLIP170 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117504

Cat. No. GTX117504

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CLIP170 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

162 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CLIP170. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176003

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CAP-Gly domain containing linker protein 1 , CLIP , CLIP-170 , CLIP170 , CYLN1 , RSN

Cellular Localization

Cytoplasm , cytoskeleton

Database

Research Area

DATA IMAGES

Anti-CLIP170 antibody [N1], N-term used in Western Blot (WB). GTX117504

GTX117504 WB Image

Sample (30 ug of whole cell lysate)
A: A431
5% SDS PAGE
GTX117504 diluted at 1:1000

Anti-CLIP170 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117504

GTX117504 ICC/IF Image

CLIP170 antibody [N1], N-term detects CLIP170 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CLIP170 stained by CLIP170 antibody [N1], N-term (GTX117504) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Barbiero I et al. Neuropharmacology 2020; 164 : 107897 Pregnenolone and pregnenolone-methyl-ether rescue neuronal defects caused by dysfunctional CLIP170 in a neuronal model of CDKL5 Deficiency Disorder.
Application : WB, ICC/IF
Reactivity : Mouse
Barbiero I et al. Hum Mol Genet 2017; The neurosteroid pregnenolone reverts microtubule derangement induced by the loss of a functional CDKL5-IQGAP1 complex.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169