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CLK3 antibody

Anti-CLK3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111451
Anti-CLK3 antibody used in Western Blot (WB). GTX111451

Cat. No. GTX111451

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CLK3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

NCI-H929

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CLK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885300

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CDC like kinase 3 , PHCLK3 , PHCLK3/152

Cellular Localization

Nucleus , Cytoplasm , Acrosome

Background

This gene encodes a protein belonging to the serine/threonine type protein kinase family. This protein is a nuclear dual-specificity kinase that regulates the intranuclear distribution of the serine/arginine-rich (SR) family of splicing factors. Two transcript variants encoding different isoforms have been found for this gene. Related pseudogenes are located on chromosomes 1 and 9. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CLK3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111451

GTX111451 ICC/IF Image

CLK3 antibody detects CLK3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CLK3 stained by CLK3 antibody (GTX111451) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CLK3 antibody used in Western Blot (WB). GTX111451

GTX111451 WB Image

CLK3 antibody detects CLK3 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CLK3 antibody (GTX111451) diluted by 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169