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CLOCK antibody [HL1099]

Recombinant antibody KO/KD Comparison Orthogonal
Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283
Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283
Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283
Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283
Anti-CLOCK antibody [HL1099] used in IHC (Paraffin sections) (IHC-P). GTX636283
Anti-CLOCK antibody [HL1099] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636283

Cat. No. GTX636283

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1099

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CLOCK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

Predict Reactivity

Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CLOCK. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909961

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

clock circadian regulator , KAT13D , bHLHe8

Cellular Localization

Nucleus,Cytoplasm,Cytoplasm, cytosol

Background

The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283

GTX636283 WB Image

KO/KD

Wild-type (WT) and NME4 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CLOCK antibody [HL1099] (GTX636283) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283

GTX636283 WB Image

Comparison

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with CLOCK antibody [HL1099] (GTX636283) diluted at 1:2000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283

GTX636283 WB Image

Comparison

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with CLOCK antibody [HL1099] (GTX636283) diluted at 1:2000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283

GTX636283 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CLOCK antibody [HL1099] (GTX636283) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CLOCK antibody [HL1099] used in IHC (Paraffin sections) (IHC-P). GTX636283

GTX636283 IHC-P Image

CLOCK antibody [HL1099] detects CLOCK protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
CLOCK stained by CLOCK antibody [HL1099] (GTX636283) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CLOCK antibody [HL1099] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636283

GTX636283 ICC/IF Image

CLOCK antibody [HL1099] detects CLOCK protein by immunofluorescent analysis.
Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CLOCK stained by CLOCK antibody [HL1099] (GTX636283) diluted at 1:500.
Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations