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CLP1 antibody [N3C3]

Anti-CLP1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115518
Anti-CLP1 antibody [N3C3] used in Western Blot (WB). GTX115518
Anti-CLP1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115518

Cat. No. GTX115518

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CLP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa , HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CLP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10625506

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cleavage and polyadenylation factor I subunit 1 , HEAB , hClp1

Background

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.

Database

Research Area

DATA IMAGES

Anti-CLP1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115518

GTX115518 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HS578T xenograft, using CLP1(GTX115518) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CLP1 antibody [N3C3] used in Western Blot (WB). GTX115518

GTX115518 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
10% SDS PAGE
GTX115518 diluted at 1:1000

Anti-CLP1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115518

GTX115518 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CLP1(GTX115518) antibody at 1:200 dilution.

REFERENCE

Application Reference

Salzman DW et al. Nat Commun 2016; 7 : 10954 miR-34 activity is modulated through 5'-end phosphorylation in response to DNA damage.
Application : WB, IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169