CLPP antibody [C2C3], C-term

Name: CLPP antibody [C2C3], C-term
Brand: GeneTex
SKU: GTX104656
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-CLPP antibody [C2C3], C-term used in Western Blot (WB). GTX104656

Anti-CLPP antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104656

Anti-CLPP antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104656

Cat. No. GTX104656

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all CLPP products

View product citations for antibody GTX104656 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CLPP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240636

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caseinolytic mitochondrial matrix peptidase proteolytic subunit , DFNB81 , PRLTS3

Cellular Localization

Mitochondrion

Background

The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq]

Database

Gene ID: 8192 CLPP
UniProt: Q16740 CLPP

Research Area

DATA IMAGES

GTX104656 WB Image

Wild-type (WT) and CLPP knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CLPP antibody [C2C3], C-term (GTX104656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104656 ICC/IF Image

CLPP antibody [C2C3], C-term detects CLPP protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.
Green: CLPP protein stained by CLPP antibody [C2C3], C-term (GTX104656) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX104656 WB Image

CLPP antibody detects CLPP protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CLPP antibody (GTX104656) diluted by 1:500.

GTX104656 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H520 xenograft, using CLPP(GTX104656) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Sen B et al. Cell Mol Gastroenterol Hepatol 2019; 8 (1) : 73-94 Senescent Hepatocytes in Decompensated Liver Show Reduced UPR^MT and Its Key Player, CLPP, Attenuates Senescence In?Vitro.

Application : WB

Reactivity : Human

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REVIEW