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CLPP antibody [N2C3]

Anti-CLPP antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115070
Anti-CLPP antibody [N2C3] used in Western Blot (WB). GTX115070

Cat. No. GTX115070

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CLPP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CLPP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172432

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caseinolytic mitochondrial matrix peptidase proteolytic subunit , DFNB81 , PRLTS3

Cellular Localization

Mitochondrion

Background

The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CLPP antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115070

GTX115070 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CLPP(GTX115070) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CLPP antibody [N2C3] used in Western Blot (WB). GTX115070

GTX115070 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
12% SDS PAGE
GTX115070 diluted at 1:1000

REFERENCE

Application Reference

Hu D et al. Acta Neuropathol 2019; 137 (6) : 939-960 Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity.
Sandhu R et al. Biochem Biophys Res Commun 2019; 512 (2) : 230-235 An N-terminal Flag-tag impairs TPP1 regulation of telomerase function.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169