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CLPX antibody

Anti-CLPX antibody used in Flow cytometry (FACS). GTX53477
Anti-CLPX antibody used in Western Blot (WB). GTX53477
Anti-CLPX antibody used in IHC (Paraffin sections) (IHC-P). GTX53477

Cat. No. GTX53477

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all CLPX products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 449-477 amino acids from the C-terminal region of human CLPX.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caseinolytic mitochondrial matrix peptidase chaperone subunit , EPP2

Cellular Localization

Mitochondrion

Background

The protein encoded by this gene is part of a protease found in mitochondria. This protease is ATP-dependent and targets specific proteins for degradation. The protease consists of two heptameric rings of the CLPP catalytic subunit sandwiched between two hexameric rings of the chaperone subunit encoded by this gene. Targeted proteins are unwound by this protein and then passed on to the CLPP subunit for degradation. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-CLPX antibody used in Flow cytometry (FACS). GTX53477

GTX53477 FACS Image

FACS analysis of 293 cells using GTX53477 CLPX antibody.
Green: Primary antibody
Blue : negative control

Anti-CLPX antibody used in Western Blot (WB). GTX53477

GTX53477 WB Image

WB analysis of human heart tissue lysate using GTX53477 CLPX antibody.
Loading : 35ug per lane
Dilution : 1:1000

Anti-CLPX antibody used in IHC (Paraffin sections) (IHC-P). GTX53477

GTX53477 IHC-P Image

IHC-P analysis of human hepatocarcinoma using GTX53477 CLPX antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399