APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , HeLa , HepG2 , HCT116 , mouse brain , rat brain
Predict Reactivity
Chicken, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CLTA. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
clathrin light chain A , LCA
Cellular Localization
Cytoplasmic vesicle membrane , Membrane , coated pit
Background
Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-CLTA antibody [N1C3] used in Western Blot (WB). GTX101939](/upload/website/prouct_img/middle/GTX101939/GTX101939_40562_WB_19011506_417.jpg "Anti-CLTA antibody [N1C3] used in Western Blot (WB). GTX101939")
|
GTX101939 WB Image
Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
GTX101939 diluted at 1:1000
|
![Anti-CLTA antibody [N1C3] used in Western Blot (WB). GTX101939](/upload/website/prouct_img/middle/GTX101939/GTX101939_40562_20170518_WB_M_R_19011506_196.jpg "Anti-CLTA antibody [N1C3] used in Western Blot (WB). GTX101939")
|
GTX101939 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CLTA antibody (GTX101939) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for CLTA antibody [N1C3] (GTX101939). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CLTA antibody [N1C3] (GTX101939). Be the first to share your experience with this product.
![Anti-CLTA antibody [N1C3] used in Western Blot (WB). GTX101939](/upload/website/prouct_img/small/GTX101939/GTX101939_40562_WB_19011506_417.jpg "Anti-CLTA antibody [N1C3] used in Western Blot (WB). GTX101939")
![Anti-CLTA antibody [N1C3] used in Western Blot (WB). GTX101939](/upload/website/prouct_img/small/GTX101939/GTX101939_40562_20170518_WB_M_R_19011506_196.jpg "Anti-CLTA antibody [N1C3] used in Western Blot (WB). GTX101939")
![Anti-CLTA antibody [N1C3] used in Western Blot (WB). GTX101939](/upload/website/prouct_img/normal/GTX101939/GTX101939_40562_WB_19011506_417.jpg "Anti-CLTA antibody [N1C3] used in Western Blot (WB). GTX101939")
![Anti-CLTA antibody [N1C3] used in Western Blot (WB). GTX101939](/upload/website/prouct_img/normal/GTX101939/GTX101939_40562_20170518_WB_M_R_19011506_196.jpg "Anti-CLTA antibody [N1C3] used in Western Blot (WB). GTX101939")
