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CLTA antibody [N1C3]

Anti-CLTA antibody [N1C3] used in Western Blot (WB). GTX101939
Anti-CLTA antibody [N1C3] used in Western Blot (WB). GTX101939

Cat No. GTX101939

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
See all CLTA products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116 , mouse brain , rat brain

Predict Reactivity

Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CLTA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

clathrin light chain A , LCA

Cellular Localization

Cytoplasmic vesicle membrane , Membrane , coated pit

Background

Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-CLTA antibody [N1C3] used in Western Blot (WB). GTX101939

GTX101939 WB Image

Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
GTX101939 diluted at 1:1000

Anti-CLTA antibody [N1C3] used in Western Blot (WB). GTX101939

GTX101939 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CLTA antibody (GTX101939) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169