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CMG1 antibody, C-term

Anti-CMG1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89771
Anti-CMG1 antibody, C-term used in Western Blot (WB). GTX89771
Anti-CMG1 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89771

Cat. No. GTX89771

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μg ($399)
See all CMG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
ICC/IF Assay dependent
IHC-P 5-10μg/ml

Note :

IHC-P
Human Liver shows vesicular staining in cytoplasm of hepatocytes.
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89771-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KTIVDALHSTSGN, from the C Terminus of the protein sequence according to NP_001092692.1; NP_001092693.1; NP_079379.2; AAK77221.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

intraflagellar transport 74 , BBS20 , CCDC2 , CMG-1 , CMG1

Cellular Localization

Cytoplasmic vesicle

Background

This gene encodes a core intraflagellar transport (IFT) protein which belongs to a multi-protein complex involved in the transport of ciliary proteins along axonemal microtubules. IFT proteins are found at the base of the cilium as well as inside the cilium, where they assemble into long arrays between the ciliary base and tip. This protein, together with intraflagellar transport protein 81, binds and transports tubulin within cilia and is required for ciliogenesis. Naturally occurring mutations in this gene are associated with amyotrophic lateral sclerosis--frontotemporal dementia and Bardet-Biedl Syndrome. [provided by RefSeq, Mar 2017]

Database

Research Area

DATA IMAGES

Anti-CMG1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89771

GTX89771 IHC-P Image

IHC-P analysis of human liver using GTX89771 CMG1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-CMG1 antibody, C-term used in Western Blot (WB). GTX89771

GTX89771 WB Image

WB analysis of 293 lysate using GTX89771 CMG1 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-CMG1 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89771

GTX89771 ICC/IF Image

ICC/IF analysis of primary rat cortical neurons using GTX89771 CMG1 antibody, C-term.
Dilution : 2.5μg/ml
A: Primary antibody, B: control

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399