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CMPK1 antibody [N1C3]

Anti-CMPK1 antibody [N1C3] used in Western Blot (WB). GTX105566
Anti-CMPK1 antibody [N1C3] used in Western Blot (WB). GTX105566
Anti-CMPK1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105566

Cat No. GTX105566

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Raji , mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CMPK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cytidine/uridine monophosphate kinase 1 , CK , CMK , CMPK , UMK , UMP-CMPK , UMPK

Cellular Localization

Nucleus , Cytoplasm

Background

Uridine monophosphate (UMP)/cytidine monophosphate (CMP) kinase (EC 2.7.4.4) catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. These nucleoside diphosphates are required for cellular nucleic acid synthesis (Liou et al., 2002 [PubMed 11912132]).[supplied by OMIM]

Database

DATA IMAGES
Anti-CMPK1 antibody [N1C3] used in Western Blot (WB). GTX105566

GTX105566 WB Image

Sample(30 ug whole cell lysate)
A:Raji (GTX27908)
12% SDS PAGE
GTX105566 diluted at 1:500

Anti-CMPK1 antibody [N1C3] used in Western Blot (WB). GTX105566

GTX105566 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105566 diluted at 1:1000

Anti-CMPK1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105566

GTX105566 ICC/IF Image

CMPK1 antibody [N1C3] detects CMPK1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CMPK1 protein stained by CMPK1 antibody [N1C3] (GTX105566) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169