GeneTex
United States (US)

CMPK1 antibody [N1C3]

GTX105566_WB_19011506_410.jpg
GTX105566_39932_WB_M_brain_19011506_267.jpg
GTX105566_39932_IFA_19011506_768.jpg

Cat No. GTX105566

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Raji , mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CMPK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CMK antibody, CMPK antibody, UMK antibody, UMP-CMPK antibody, UMPK antibody, CMPK1 antibody, UMP-CMP kinase antibody, uridine monophosphate kinase antibody, uridine monophosphate/cytidine monophosphate kinase antibody, UMP/CMP kinase antibody, deoxycytidylate kinase antibody, cytidylate kinase antibody, "cytidine monophosphate (UMP-CMP) kinase 1, cytosolic antibody"

Cellular Localization

Nucleus , Cytoplasm

Background

Uridine monophosphate (UMP)/cytidine monophosphate (CMP) kinase (EC 2.7.4.4) catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. These nucleoside diphosphates are required for cellular nucleic acid synthesis (Liou et al., 2002 [PubMed 11912132]).[supplied by OMIM]

Database

DATA IMAGES
GTX105566_WB_19011506_410.jpg

GTX105566 WB Image

Sample(30 ug whole cell lysate)
A:Raji (GTX27908)
12% SDS PAGE
GTX105566 diluted at 1:500

GTX105566_39932_WB_M_brain_19011506_267.jpg

GTX105566 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105566 diluted at 1:1000

GTX105566_39932_IFA_19011506_768.jpg

GTX105566 ICC/IF Image

CMPK1 antibody [N1C3] detects CMPK1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CMPK1 protein stained by CMPK1 antibody [N1C3] (GTX105566) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169