GeneTex
United States (US)

CNOT3 antibody [C2C3], C-term

GTX115005_WB_18121410_833.jpg
GTX115005_40240_IFA_18121410_941.jpg
GTX115005_40240_IHC_18121410_721.jpg

Cat No. GTX115005

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , Jurkat , HeLa

Predict Reactivity

Mouse, Rat, Rabbit, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CNOT3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ccr4-Not Transcription Complex Subunit 3,Leng2,Not3,Not3H,Cnot3

Cellular Localization

Cytoplasm , Nucleus

Background

The CCR4-NOT complex functions as general transcription regulation complex.

Database

Research Area

DATA IMAGES
GTX115005_WB_18121410_833.jpg

GTX115005 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX115005 diluted at 1:3000

GTX115005_40240_IFA_18121410_941.jpg

GTX115005 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CNOT3(GTX115005) antibody at 1:200 dilution.

GTX115005_40240_IHC_18121410_721.jpg

GTX115005 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using CNOT3(GTX115005) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169