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CNOT4 antibody

Anti-CNOT4 antibody used in Western Blot (WB). GTX85379

Cat. No. GTX85379

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($409)
See all CNOT4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

A549 Cell Lysate(GTX27910)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CNOT4 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of the human CNOT4.The immunogen is located within amino acids 70 - 120 of CNOT4.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10723837

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CCR4-NOT transcription complex subunit 4 , CLONE243 , NOT4 , NOT4H

Cellular Localization

Cytoplasm,Nucleus

Background

CNOT4 is a component of the CCR4-NOT transcription complex, a complex that is implicated in the repression of RNA polymerase II transcription. In the CCR4-NOT complex, CNOT4 acts as an E3 ubiquitin-protein ligase and interacts with a subset of E2 ubiquitin-conjugating enzymes through a unique C4C4 RING domain. This E3 ligase activity was shown to be dependent on the selective and specific interaction with the ubiquitin conjugating enzyme UbcH5B. In yeast, mutations in CNOT4 that prevented its interaction with the UbcH5B homolog UBC4 caused increased sensitivity to hydroxyurea, heat shock, and hygromycin B, suggesting that CNOT4 and UbcH5B are involved in stress response in vivo. Multiple isoforms of CNOT4 are known to exist.

Database

Research Area

DATA IMAGES

Anti-CNOT4 antibody used in Western Blot (WB). GTX85379

GTX85379 WB Image

WB analysis of A549 cell lysate using GTX85379 CNOT4 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

Application Reference

Hagkarim NC et al. J Virol 2018; (Epub) Degradation of a novel DNA damage response protein, tankyrase 1 binding protein 1 (Tab182), following adenovirus infection.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409