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CNOT8 antibody [HL2586]

Recombinant antibody Overexpression
Anti-CNOT8 antibody [HL2586] used in Western Blot (WB). GTX639037
Anti-CNOT8 antibody [HL2586] used in Western Blot (WB). GTX639037
Anti-CNOT8 antibody [HL2586] used in Western Blot (WB). GTX639037
Anti-CNOT8 antibody [HL2586] used in Western Blot (WB). GTX639037
Anti-CNOT8 antibody [HL2586] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639037

Cat. No. GTX639037

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2586

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all CNOT8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Product Note

This antibody is specific for human CNOT8 protein, and it does not cross react with human CNOT7 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human CNOT8.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CCR4-NOT transcription complex subunit 8 , CAF1 , CALIF , Caf1b , POP2 , hCAF1

Cellular Localization

Cytoplasm,Nucleus

Background

Enables poly(A)-specific ribonuclease activity. Involved in exonucleolytic catabolism of deadenylated mRNA and positive regulation of cell population proliferation. Located in nucleus. Part of CCR4-NOT complex. [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-CNOT8 antibody [HL2586] used in Western Blot (WB). GTX639037

GTX639037 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (GTX639037) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CNOT8 antibody [HL2586] used in Western Blot (WB). GTX639037

GTX639037 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (GTX639037) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CNOT8 antibody [HL2586] used in Western Blot (WB). GTX639037

GTX639037 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (GTX639037) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CNOT8 antibody [HL2586] used in Western Blot (WB). GTX639037

GTX639037 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (GTX639037) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CNOT8 antibody [HL2586] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639037

GTX639037 ICC/IF Image

CNOT8 antibody [HL2586] detects CNOT8 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CNOT8 stained by CNOT8 antibody [HL2586] (GTX639037) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations