Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (GTX639037) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CNOT8 antibody [HL2586] detects CNOT8 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CNOT8 stained by CNOT8 antibody [HL2586] (GTX639037) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (GTX639037) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (GTX639037) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (GTX639037) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.