GeneTex

  • Country / Location Selection

United States (US)

CNOT8 antibody

Anti-CNOT8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105674
Anti-CNOT8 antibody used in Western Blot (WB). GTX105674

Cat. No. GTX105674

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CNOT8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

SK-N-SH

Product Note

Based on internal testing result, this antibody may cross react with CNOT7.

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CNOT8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170629

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CCR4-NOT transcription complex subunit 8 , CAF1 , CALIF , Caf1b , POP2 , hCAF1

Cellular Localization

Cytoplasm , Nucleus

Database

Research Area

DATA IMAGES

Anti-CNOT8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105674

GTX105674 ICC/IF Image

CNOT8 antibody detects CNOT8 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CNOT8 protein stained by CNOT8 antibody (GTX105674) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-CNOT8 antibody used in Western Blot (WB). GTX105674

GTX105674 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody (GTX105674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Submit a Reference

REVIEW

There are currently no reviews for CNOT8 antibody (GTX105674). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169