GeneTex
United States (US)

CNPase antibody

KO/KD
GTX103954_40128_20170531_IHC-Fr_M_19011506_106.jpg
GTX103954_40128_20170629_WB_KO_watermark_19011506_896.jpg
GTX103954_40128_IHC_19011506_751.jpg
GTX103954_WB_19011506_213.jpg
GTX103954_40128_20161020_WB_M_R_19011506_392.jpg
GTX103954_40128_42886_IHC-Fr_M_19011506_307.jpg

Cat No. GTX103954

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa , Molt-4 , mouse brain , rat brain

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CNPase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CNP1 antibody, CNP antibody, CNPase antibody, 2',3'-cyclic-nucleotide 3'-phosphodiesterase antibody, 2', 3' cyclic nucleotide 3' phosphohydrolase antibody, 2',3'-cyclic nucleotide 3' phosphodiesterase antibody

Cellular Localization

Membrane , Melanosome

Database

Research Area

DATA IMAGES
GTX103954_40128_20170531_IHC-Fr_M_19011506_106.jpg

GTX103954 IHC-Fr Image

CNPase antibody detects CNPase protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: CNPase protein stained by CNPase antibody (GTX103954) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX103954_40128_20170629_WB_KO_watermark_19011506_896.jpg

GTX103954 WB Image

KO/KD

Wild-type (WT) and CNPase knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CNPase antibody (GTX103954) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX103954_40128_IHC_19011506_751.jpg

GTX103954 IHC-P Image

CNP antibody detects CNP protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
CNP antibody (GTX103954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103954_WB_19011506_213.jpg

GTX103954 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
10% SDS PAGE
GTX103954 diluted at 1:1000

GTX103954_40128_20161020_WB_M_R_19011506_392.jpg

GTX103954 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CNP antibody (GTX103954) diluted at 1:5000.

GTX103954_40128_42886_IHC-Fr_M_19011506_307.jpg

GTX103954 IHC-Fr Image

CNPase antibody detects CNPase protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: CNPase protein stained by CNPase antibody (GTX103954) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169