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CNPase antibody

KO/KD
Anti-CNPase antibody used in IHC (Frozen sections) (IHC-Fr). GTX103954
Anti-CNPase antibody used in Western Blot (WB). GTX103954
Anti-CNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX103954
Anti-CNPase antibody used in Western Blot (WB). GTX103954
Anti-CNPase antibody used in Western Blot (WB). GTX103954
Anti-CNPase antibody used in IHC (Frozen sections) (IHC-Fr). GTX103954

Cat No. GTX103954

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all CNPase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa , Molt-4 , mouse brain , rat brain

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CNPase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2',3'-cyclic nucleotide 3' phosphodiesterase , CNP1

Cellular Localization

Membrane , Melanosome

Database

Research Area

DATA IMAGES

Anti-CNPase antibody used in IHC (Frozen sections) (IHC-Fr). GTX103954

GTX103954 IHC-Fr Image

CNPase antibody detects CNPase protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: CNPase protein stained by CNPase antibody (GTX103954) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-CNPase antibody used in Western Blot (WB). GTX103954

GTX103954 WB Image

KO/KD

Wild-type (WT) and CNPase knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CNPase antibody (GTX103954) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX103954

GTX103954 IHC-P Image

CNP antibody detects CNP protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
CNP antibody (GTX103954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CNPase antibody used in Western Blot (WB). GTX103954

GTX103954 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
10% SDS PAGE
GTX103954 diluted at 1:1000

Anti-CNPase antibody used in Western Blot (WB). GTX103954

GTX103954 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CNP antibody (GTX103954) diluted at 1:5000.

Anti-CNPase antibody used in IHC (Frozen sections) (IHC-Fr). GTX103954

GTX103954 IHC-Fr Image

CNPase antibody detects CNPase protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: CNPase protein stained by CNPase antibody (GTX103954) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.

REFERENCE

Application Reference

Chen SY et al. Stem Cells Transl Med 2020; (Epub) Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169