Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CNTN5 antibody [C1C3] (GTX121400) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CNTN5 antibody [C1C3] (GTX121400) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.