GeneTex
United States (US)

COASY antibody [N3C3-3]

GTX107934_WB_19011507_408.jpg
GTX107934_40009_20150417_IHC_M_2_19011507_241.jpg
GTX107934_IHC_19011507_519.jpg
GTX107934_IFA_19011507_995.jpg
GTX107934_40009_WB_M_liver_19011507_655.jpg
GTX107934_40009_20150417_IHC_M_19011507_257.jpg

Cat No. GTX107934

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse liver

Predict Reactivity

Rat, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COASY. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Coenzyme A Synthase,Dpck,Nbia6,Nbp,Ppat,Ukr1,Pov-2,Coasy

Cellular Localization

Cytoplasm

Background

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX107934_WB_19011507_408.jpg

GTX107934 WB Image

Sample (30μg whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX107934 diluted at 1:1000

GTX107934_40009_20150417_IHC_M_2_19011507_241.jpg

GTX107934 IHC-P Image

CoA synthase antibody [N3C3-3] detects CoA synthase protein at cytoplasm in mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
CoA synthase antibody [N3C3-3] (GTX107934) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107934_IHC_19011507_519.jpg

GTX107934 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using CoA synthase(GTX107934) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107934_IFA_19011507_995.jpg

GTX107934 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CoA synthase(GTX107934) antibody at 1:200 dilution.

GTX107934_40009_WB_M_liver_19011507_655.jpg

GTX107934 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX107934 diluted at 1:3000

GTX107934_40009_20150417_IHC_M_19011507_257.jpg

GTX107934 IHC-P Image

CoA synthase antibody [N3C3-3] detects CoA synthase protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CoA synthase antibody [N3C3-3] (GTX107934) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169