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COBRA1 antibody [F7E4]

Anti-COBRA1 antibody [F7E4] used in Western Blot (WB). GTX82722

Cat. No. GTX82722

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 F7E4

Isotype

 IgG1

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($499)
See all COBRA1 products

APPLICATION

Application Note

Optimal working dilutions should be determined experimentally by the end user.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM HEPES, 150mM NaCl, 0.01% BSA, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length human COBRA1 purified from E. coli

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_11164999

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

negative elongation factor complex member B , COBRA1 , NELF-B

Cellular Localization

Nucleus

Background

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]

Database

DATA IMAGES

Anti-COBRA1 antibody [F7E4] used in Western Blot (WB). GTX82722

GTX82722 WB Image

Western blot of rat neonatal brain lysate showing specific immunolabeling of the ~62k COBRA1 protein using COBRA1 (NELF-B) antibody [F7E4] (GTX82722).

REFERENCE

Application Reference

Menard M et al. PLoS Biol 2018; 16 (5) : e2002912 Hey1- and p53-dependent TrkC proapoptotic activity controls neuroblastoma growth.
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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 499