GeneTex
United States (US)

COBRA1 antibody

GTX102043_39631_IHC_2_19011506_712.jpg
GTX102043_39631_IFA_19011506_777.jpg
GTX102043_WB_19011506_892.jpg
GTX102043_39631_IHC_19011506_508.jpg
GTX102043_39631_IHC_M_19011506_548.jpg

Cat No. GTX102043

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Rat, Zebrafish, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COBRA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Negative Elongation Factor Complex Member B,Cobra1,Nelf-B,Nelfb

Cellular Localization

Nucleus

Background

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM]

Database

DATA IMAGES
GTX102043_39631_IHC_2_19011506_712.jpg

GTX102043 IHC-P Image

COBRA1 antibody detects COBRA1 protein at nucleus on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
COBRA1 antibody (GTX102043) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102043_39631_IFA_19011506_777.jpg

GTX102043 ICC/IF Image

Immunofluorescence analysis of methanol-fixed H1299, using COBRA1(GTX102043) antibody at 1:500 dilution.

GTX102043_WB_19011506_892.jpg

GTX102043 WB Image

Sample (30 ug of whole cell lysate)

A: H1299

5% SDS PAGE

GTX101827 diluted at 1:1000

GTX102043_39631_IHC_19011506_508.jpg

GTX102043 IHC-P Image

COBRA1 antibody detects COBRA1 protein at nucleus on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
COBRA1 antibody (GTX102043) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102043_39631_IHC_M_19011506_548.jpg

GTX102043 IHC-P Image

COBRA1 antibody detects COBRA1 protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
COBRA1 antibody (GTX102043) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169