Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL11A2 antibody [GT473] (GTX629740) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

COL11A2 antibody [GT473] detects COL11A2 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COL11A2 protein stained by COL11A2 antibody [GT473] (GTX629740) diluted at 1:500.
Blue: Hoechst 33342 staining.

COL11A2 antibody [GT473] detects COL11A2 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: COL11A2 protein stained by COL11A2 antibody [GT473] (GTX629740) diluted at 1:500.
Blue: Hoechst 33342 staining.

COL11A2 antibody [GT473] detects COL11A2 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human COL11A2-transfected 293T cells (N-terminal fragment of COL11A2 isoform 1)
12 % SDS-PAGE
COL11A2 antibody [GT473] (GTX629740) dilution: 1:5000