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COL17A1 antibody, Internal

Anti-COL17A1 antibody, Internal used in Flow cytometry (FACS). GTX80563
Anti-COL17A1 antibody, Internal used in Western Blot (WB). GTX80563

Cat. No. GTX80563

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human
Package
400 μl ($399)
See all COL17A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
FACS 1:25
Not tested in other applications.

Calculated MW

150 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 475-504 amino acids from the Central region of human COL17A1.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type XVII alpha 1 chain , BA16H23.2 , BP180 , BPA-2 , BPAG2 , ERED , LAD-1

Background

This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-COL17A1 antibody, Internal used in Flow cytometry (FACS). GTX80563

GTX80563 FACS Image

FACS analysis of A431 cells using GTX80563 COL17A1 antibody, Internal.
Green : COL17A1
Blue : Isotype control
Fixation : 2% paraformaldehyde (PFA) (10 min)
Permeabilization : 90% methanol (10 min)
Dilution : 1:25

Anti-COL17A1 antibody, Internal used in Western Blot (WB). GTX80563

GTX80563 WB Image

WB analysis of various samples using GTX80563 COL17A1 antibody, Internal.
Lane 1: A2058 whole cell lysate
Lane 2: A375 whole cell lysate
Lane 3: HL-60 whole cell lysate
Lane 4: MCF-7 whole cell lysate
Loading : 20 μg per lane
Dilution : 1:2000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399