GeneTex
  • Country / Location Selection

United States (US)

COL1A1 antibody [N1N2], N-term

Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731

Cat No. GTX112731

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

SK-N-SH , SK-N-AS , NIH3T3

Predict Reactivity

Bovine, Dog, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human COL1A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721155

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

collagen type I alpha 1 chain , EDSARTH1 , EDSC , OI1 , OI2 , OI3 , OI4

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]

Database

Research Area

DATA IMAGES

Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COL1A1 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX112731

GTX112731 IP Image

Immunoprecipitation of COL1A1 protein from SK-N-AS whole cell extracts using 5 μg of COL1A1 antibody [N1N2], N-term (GTX112731).
Western blot analysis was performed using COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731

GTX112731 WB Image

COL1A1 antibody detects COL1A1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody (GTX112731) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COL1A1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112731

GTX112731 ICC/IF Image

COL1A1 antibody [N1N2], N-term detects COL1A1 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COL1A1 stained by COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731

GTX112731 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects secreted COL1A1 protein in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects secreted COL1A1 protein in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

COL1A1 antibody [N1N2], N-term Cat No. GTX112731

Rating ( Average 5 based on 4 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Cell Mol Med in 2020.PMID: 31957232
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Cell Mol Med in 2020.PMID: 31957232
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Cell Mol Med in 2020.PMID: 31957232
Date : Anonymous submitted on 02-Jul-2014
Score :
Application Tested : WB
Sample Species : Ms
Sample : NIH3T3 cells
Amount used : 15μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf