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COL1A1 antibody

Anti-COL1A1 antibody used in Western Blot (WB). GTX82720
Anti-COL1A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX82720

Cat. No. GTX82720

Reference 3
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Sheep
Package
100 μl ($499)
See all COL1A1 products

APPLICATION

Application Note

We recommend the following starting dilutions:
For WB: Use at a dilution of 1:1000.
For IHC: Use at a dilution of 1:100.
Optimal dilutions should be determined by the end user.

Calculated MW

139 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen corresponding to a region within amino acids 1200 and 1250 of human collagen I α1 telopeptide.

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type I alpha 1 chain , EDSARTH1 , EDSC , OI1 , OI2 , OI3 , OI4

Background

This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]

Database

Research Area

DATA IMAGES

Anti-COL1A1 antibody used in Western Blot (WB). GTX82720

GTX82720 WB Image

Western blot of rat lung lysate showing specific immunolabeling of the ~140k collagen protein using COL1A1 antibody (GTX82720).

Anti-COL1A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX82720

GTX82720 IHC-P Image

Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (red) that has formed fibrils in the extracellular matrix

REFERENCE

Application Reference

Lin Z et al. Int J Mol Sci 2023; 24 (24) Therapeutic Potential of Adipose-Derived Stem Cell-Conditioned Medium and Extracellular Vesicles in an In Vitro Radiation-Induced Skin Injury Model.
Zhang X et al. Theranostics 2022; 12 (2) : 530-541 Modulation of H4K16Ac levels reduces pro-fibrotic gene expression and mitigates lung fibrosis in aged mice.
Application : WB
Reactivity : Human
Hyerin Jung et al. J Clin Med 2021; 10 (14) Restoration of Osteogenesis by CRISPR/Cas9 Genome Editing of the Mutated COL1A1 Gene in Osteogenesis Imperfecta.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

COL1A1 antibody Cat. No. GTX82720

Rating
( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-COL1A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82720
Date : Anonymous submitted on 14-Dec-2020
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : MC3T3-E1 cells
Blocking : 1% BSA in PBS, 25°C, 0.5Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : 1% TritonX-100 in PBS
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : Day 9 after the induction of osteoblastic differentiation
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SDS
PBS.pdf
Package List Price ($)
$ 499