*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Rat, Rabbit, Bovine, Dog, Chicken, Pig, Rhesus Monkey(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.51 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the C-terminus region of human COL1A2. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Collagen Type I Alpha 2 Chain,Edsarth2,Edscv,Oi4,Col1A2
Secreted , extracellular space , extracellular matrix
This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]
GTX102996 ICC/IF Image
COL1A2 antibody [C2C3], C-term detects COL1A2 protein at cytoplasm by immunofluorescent analysis.
Sample: NIH-3T3 cells were fixed in ice-cold MeOH for 5 min.
Green: COL1A2 protein stained by COL1A2 antibody [C2C3], C-term (GTX102996) diluted at 1:500.
Blue: Hoechst 33342 staining.
GTX102996 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [C2C3], C-term (GTX102996) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.