COL1A2 antibody [C2C3], C-term

Name: COL1A2 antibody [C2C3], C-term
Brand: GeneTex
SKU: GTX102996
Price: 399.00 USD

Anti-COL1A2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102996

Anti-COL1A2 antibody [C2C3], C-term used in Western Blot (WB). GTX102996

Cat. No. GTX102996

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human, Mouse, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all COL1A2 products

View product citations for antibody GTX102996 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

129 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human COL1A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950011

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type I alpha 2 chain , EDSARTH2 , EDSCV , OI4

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]

Database

Gene ID: 1278 COL1A2
UniProt: P08123 COL1A2

Research Area

DATA IMAGES

GTX102996 ICC/IF Image

COL1A2 antibody [C2C3], C-term detects COL1A2 protein at cytoplasm by immunofluorescent analysis.
Sample: NIH-3T3 cells were fixed in ice-cold MeOH for 5 min.
Green: COL1A2 protein stained by COL1A2 antibody [C2C3], C-term (GTX102996) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX102996 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Col1a2 antibody (GTX102996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102996 WB Image

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [C2C3], C-term (GTX102996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102996 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [C2C3], C-term (GTX102996) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.