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COL1A2 antibody [HL2048]

Recombinant antibody Orthogonal Overexpression
Anti-COL1A2 antibody [HL2048] used in IHC (Paraffin sections) (IHC-P). GTX637952
Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952
Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952
Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952

Cat. No. GTX637952

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2048

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Dog
Package
100 μl ($399),
25 μl ($169)
See all COL1A2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

129 kDa. ( Note )

Product Note

This antibody is specific for human COL1A2 protein, and it does not cross react with human COL1A1, COL2A1, and COL3A1 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human COL1A2

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type I alpha 2 chain , EDSARTH2 , EDSCV , OI4

Cellular Localization

Secreted, extracellular space, extracellular matrix

Background

This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]

Database

Research Area

DATA IMAGES

Anti-COL1A2 antibody [HL2048] used in IHC (Paraffin sections) (IHC-P). GTX637952

GTX637952 IHC-P Image

COL1A2 antibody [HL2048] detects secreted COL1A2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
COL1A2 stained by COL1A2 antibody [HL2048] (GTX637952) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952

GTX637952 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2048] (GTX637952) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952

GTX637952 WB Image

Orthogonal

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2048] (GTX637952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COL1A2 antibody [HL2048] used in Western Blot (WB). GTX637952

GTX637952 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2048] (GTX637952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations