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COL4A1 antibody [HL1351]

Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771
Anti-COL4A1 antibody [HL1351] used in Western Blot (WB). GTX636771
Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771
Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771

Cat No. GTX636771

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1351

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus of human COL4A1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910074

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

collagen type IV alpha 1 chain , BSVD , RATOR

Background

This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771

GTX636771 IHC-P Image

COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
COL4A1 stained by COL4A1 antibody [HL1351] (GTX636771) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COL4A1 antibody [HL1351] used in Western Blot (WB). GTX636771

GTX636771 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL4A1 antibody [HL1351] (GTX636771) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771

GTX636771 IHC-P Image

COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
COL4A1 stained by COL4A1 antibody [HL1351] (GTX636771) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771

GTX636771 IHC-P Image

COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
COL4A1 stained by COL4A1 antibody [HL1351] (GTX636771) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for COL4A1 antibody [HL1351] (GTX636771). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for COL4A1 antibody [HL1351] (GTX636771). Be the first to share your experience with this product.
Package List Price ($)
$ 379
$ 169
Custom Conjugations