APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus of human COL4A1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2910074
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
collagen type IV alpha 1 chain , BSVD , RATOR
Background
This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Database
Research Area
DATA IMAGES
![Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771](/templates/default/js/lightbox/images/loading.gif "Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771")
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GTX636771 IHC-P Image
COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
COL4A1 stained by COL4A1 antibody [HL1351] (GTX636771) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636771 ICC/IF Image
COL4A1 antibody [HL1351] detects COL4A1 protein at vesicle by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: COL4A1 stained by COL4A1 antibody [HL1351] (GTX636771) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636771 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL4A1 antibody [HL1351] (GTX636771) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636771 IHC-P Image
COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
COL4A1 stained by COL4A1 antibody [HL1351] (GTX636771) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636771 IHC-P Image
COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
COL4A1 stained by COL4A1 antibody [HL1351] (GTX636771) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
REFERENCE
There are currently no references for COL4A1 antibody [HL1351] (GTX636771). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for COL4A1 antibody [HL1351] (GTX636771). Be the first to share your experience with this product.
![Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771](/upload/website/prouct_img/small/GTX636771/GTX636771_44641_20220506_IHC-P_M_22061601_831.jpg "Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771")
![Anti-COL4A1 antibody [HL1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636771](/upload/website/prouct_img/small/GTX636771/GTX636771_44641_20221007_ICC_IF_22102723_693.webp "Anti-COL4A1 antibody [HL1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636771")
![Anti-COL4A1 antibody [HL1351] used in Western Blot (WB). GTX636771](/upload/website/prouct_img/small/GTX636771/GTX636771_44641_20220408_WB_22041900_206.jpg "Anti-COL4A1 antibody [HL1351] used in Western Blot (WB). GTX636771")
![Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771](/upload/website/prouct_img/small/GTX636771/GTX636771_T-44585_20220513_IHC-P_22053019_429.jpg "Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771")
![Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771](/upload/website/prouct_img/small/GTX636771/GTX636771_44641_20220506_IHC-P_M_1_22061601_326.jpg "Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771")
![Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771](/upload/website/prouct_img/normal/GTX636771/GTX636771_44641_20220506_IHC-P_M_22061601_831.jpg "Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771")
![Anti-COL4A1 antibody [HL1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636771](/upload/website/prouct_img/normal/GTX636771/GTX636771_44641_20221007_ICC_IF_22102723_693.webp "Anti-COL4A1 antibody [HL1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636771")
![Anti-COL4A1 antibody [HL1351] used in Western Blot (WB). GTX636771](/upload/website/prouct_img/normal/GTX636771/GTX636771_44641_20220408_WB_22041900_206.jpg "Anti-COL4A1 antibody [HL1351] used in Western Blot (WB). GTX636771")
![Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771](/upload/website/prouct_img/normal/GTX636771/GTX636771_T-44585_20220513_IHC-P_22053019_429.jpg "Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771")
![Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771](/upload/website/prouct_img/normal/GTX636771/GTX636771_44641_20220506_IHC-P_M_1_22061601_326.jpg "Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771")