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COL4A1 antibody [HL1351]

Recombinant antibody Orthogonal
Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771
Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771
Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771
Anti-COL4A1 antibody [HL1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636771
Anti-COL4A1 antibody [HL1351] used in Western Blot (WB). GTX636771

Cat. No. GTX636771

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1351

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all COL4A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus of human COL4A1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910074

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type IV alpha 1 chain , BSVD , RATOR

Background

This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771

GTX636771 IHC-P Image

COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
COL4A1 stained by COL4A1 antibody [HL1351] (GTX636771) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771

GTX636771 IHC-P Image

COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
COL4A1 stained by COL4A1 antibody [HL1351] (GTX636771) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COL4A1 antibody [HL1351] used in IHC (Paraffin sections) (IHC-P). GTX636771

GTX636771 IHC-P Image

COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
COL4A1 stained by COL4A1 antibody [HL1351] (GTX636771) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COL4A1 antibody [HL1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636771

GTX636771 ICC/IF Image

COL4A1 antibody [HL1351] detects COL4A1 protein at vesicle by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COL4A1 stained by COL4A1 antibody [HL1351] (GTX636771) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-COL4A1 antibody [HL1351] used in Western Blot (WB). GTX636771

GTX636771 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL4A1 antibody [HL1351] (GTX636771) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations