GeneTex
United States (US)

COL4A3BP (aa396-411) antibody, Internal

GTX88545_2225_WB_18121411_818.jpg

Cat No. GTX88545

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88545-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_005704.1; NP_112729.1).

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-KREDSWQKRLDKETEK, from the internal region of the protein sequence according to NP_005704.1; NP_112729.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GPBP Antibody , STARD11 Antibody , CERTL Antibody , COL4A3BP Antibody , CERT Antibody

Cellular Localization

Preferentially localized to the Golgi apparatus.

Background

This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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Database

DATA IMAGES
GTX88545_2225_WB_18121411_818.jpg

GTX88545 WB Image

WB analysis of human heart lysate using GTX88545 COL4A3BP (aa396-411) antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329