APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse white adipose , HepG2 , Molt-4 , Raji
Predict Reactivity
Rat, Bovine, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human COL4A3BP. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950014
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
collagen type IV alpha 3 binding protein , CERT , CERTL , GPBP , MRD34 , STARD11
Cellular Localization
Cytoplasm , Golgi apparatus , Endoplasmic reticulum
Background
This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX108337 ICC/IF Image
Immunofluorescence analysis of methanol-fixed A431, using CERT(GTX108337) antibody at 1:200 dilution.
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GTX108337 WB Image
Sample (50 ug of whole cell lysate) A: Mouse white adipose 7.5% SDS PAGE GTX108337 diluted at 1:1000
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GTX108337 IHC-P Image
COL4A3BP antibody [C3], C-term detects COL4A3BP protein at cytoplasm on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. COL4A3BP antibody [C3], C-term (GTX108337) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX108337 WB Image
Sample(30 μg of whole cell lysate) A:Hep G2(GTX27900) B:MOLT4(GTX27912) C:Raji(GTX27908) 12% SDS PAGE GTX108337 diluted at 1:500
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REFERENCE
There are currently no references for COL4A3BP antibody [C3], C-term (GTX108337). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for COL4A3BP antibody [C3], C-term (GTX108337). Be the first to share your experience with this product.