GeneTex
United States (US)

COL4A3BP antibody [C3], C-term

GTX108337_39757_IHC_19011507_561.jpg
GTX108337_39757_IFA_19011507_867.jpg
GTX108337_WB_19011507_516.jpg
GTX108337_39757_WB_M_White adipose_19011720_355.jpg

Cat No. GTX108337

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

HepG2 , Molt-4 , Raji , mouse white adipose tissue

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human COL4A3BP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CERT antibody, CERTL antibody, FLJ20597 antibody, GPBP antibody, STARD11 antibody, COL4A3BP antibody, hCERT antibody, StAR-related lipid transfer (START) domain containing 11 antibody, ceramide transfer protein antibody, collagen type IV alpha-3-binding protein antibody, lipid-transfer protein CERTL antibody, ceramide transporter antibody, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein antibody

Cellular Localization

Cytoplasm , Golgi apparatus , Endoplasmic reticulum

Background

This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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Database

DATA IMAGES
GTX108337_39757_IHC_19011507_561.jpg

GTX108337 IHC-P Image

COL4A3BP antibody [C3], C-term detects COL4A3BP protein at cytoplasm on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
COL4A3BP antibody [C3], C-term (GTX108337) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108337_39757_IFA_19011507_867.jpg

GTX108337 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using CERT(GTX108337) antibody at 1:200 dilution.

GTX108337_WB_19011507_516.jpg

GTX108337 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
B:MOLT4(GTX27912)
C:Raji(GTX27908)
12% SDS PAGE
GTX108337 diluted at 1:500

GTX108337_39757_WB_M_White adipose_19011720_355.jpg

GTX108337 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse white adipose
7.5% SDS PAGE
GTX108337 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169